J.P. Euzéby : Dictionnaire de Bactériologie Vétérinaire

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Créé le 30 janvier 2008

 

Identification des Brucella spp., de Ochrobactrum anthropi et de Ochrobactrum intermedium.
Résultats obtenus avec le système MICRONAUT (Merlin microbiological diagnostics company)

 

Le tableau présenté ci-dessous est la copie conforme du tableau publié à la page 377 de l'article de Scholz et al. : SCHOLZ (H.C.), HUBALEK (Z.), SEDLÁČEK (I.), VERGNAUD (G.), TOMASO (H.), AL DAHOUK (S.), MELZER (F.), KÄMPFER (P.), NEUBAUER (H.), CLOECKAERT (A.), MAQUART (M.), ZYGMUNT (M.S.), WHATMORE (A.M.), FALSEN (E.), BAHN (P.), GÖLLNER (C.), PFEFFER (M.), HUBER (B.), BUSSE (H.J.) and NÖCKLER (K.): Brucella microti sp. nov., isolated from the common vole Microtus arvalis. Int. J. Syst. Evol. Microbiol., 2008, 58, 375-382.

Il est publié avec l'autorisation du Comité Editorial de IJSEM (Society for General Microbiology) [Published with permission of the IJSEM Editorial Office (Society for General Microbiology)]


Table 1. Differentiating physiological reactions of B. microti sp. nov. in comparison with other Brucella and Ochrobactrum species

Taxa: 1, B. microti CCM 4915T; 2, B. microti CCM 4916; 3, B. abortus strains: 544T (=NCTC 10093T) (bv. 1), 86/8/59 (=NCTC 10501) (bv. 2), Tulya (=NCTC 10502) (bv. 3), 292 (=NCTC 10503) (bv. 4), B3196 (=NCTC 10504) (bv. 5), 870 (=NCTC 10505) (bv. 6), 63175 (=NCTC 10506) (bv. 7), C68 (=NCTC 10507) (bv. 9); 4, B. melitensis strains: 16MT (=NCTC 10094T) (bv. 1), 63/9 (=NCTC 10508) (bv. 2), Ether (=NCTC 10509) (bv. 3); 5, B . suis strains: 1330 (=NCTC 10316) (bv. 1), Thomsen (=NCTC 10510) (bv. 2), 686 (=NCTC 10511) (bv. 3), Reference 40 (bv. 4), Reference 513 (bv. 5); 6, B. ovis strain: 63/290T (=NCTC 10512T); 7, B. canis strain: RM6/66T (=NCTC 10854T); 8, B. neotomae strain: 5K33T (=NCTC 10084T); 9, B. pinnipedialis NCTC 12890T; 10, B. ceti NCTC 12891T; 11, O. anthropi LMG 3331T; 12, O. intermedium LMG 3301T. oNP, o-nitrophenyl; pNP, p-nitrophenyl; +, positive (QO2N values >70); –, negative (QO2N values <50); V, variable (QO2N values >50 and <70).

 

Compound 1 2 3 4 5 6 7 8 9 10 11 12 Substrate class
L-Glutamine + + – – – – – – – – + + Amino acid
L-Histidine hydrochloride + + – – – – – – – – + + Amino acid
D-Histidine + + – – – – – – – – + + Amino acid
L-Citrulline + + – – – – – – – – + + Amino acid
DAPI + + – – – – – – – – + + Amino acid
Sarcosine hydrochloride + + – – – – – – – – + + Amino acid derivative
Fumaric acid + + – – – – – – – – + + Organic acid
Succinic acid + + – – – – – – – – + + Organic acid
{alpha}-Ketoglutaric acid + + – – – – – – – – + + Organic acid
Itaconic acid + + – – – – – – – – – – Organic acid
Mesaconic acid + + – – – – – – – – – – Organic acid
Glutaric acid + + – – – – – – – – + + Organic acid
Arginine p-nitroanilide + + – – – – – – – – V + Amino peptidase
pNP N-acetyl-β-D-galactosaminide (pH 7.5) + + – – – – – – – – V + Esterase
oNP N-acetyl-{alpha}-D-galactosaminide (pH 7.5) + + – – – – – – – – + + Esterase
oNP N-acetyl-β-D-galactosaminide (pH 7.5) + + – – – – – – – – + + Esterase
pNP N-acetyl-β-D-glucosaminide (pH 7.5) + + – – – – – – – – + + Esterase
pNP N-acetyl-L-thio-β-D-glucosaminide (pH 7.5) V + – – – – – – – – + + Esterase
4-nitro {alpha}-D-maltoheptaose-4,6,o-ethylidene (pH 7.5) + + – – – – – – – – + + Esterase
pNP N-acetyl-β-D-glucosamide (pH 5.5) + + – – – – – – – – + – Esterase
pNP {alpha}-L-arabinopyranoside (pH 7.5) + + – – – – – – – – + + Glucosidase
oNP β-D-xylopyranoside (pH 7.5) + + – – – – – – – – + + Glucosidase
pNP L-thio-β-D-galactopyranoside (pH 7.5) + + – – – – – – – – + + Glucosidase
2-Methoxy-4-(2-nitrovinyl)-phenyl β-D-galactopyranoside + + – – – – – – – – + + Glucosidase
pNP β-L-fucopyranoside (pH 7.5) + + – – – – – – – – + + Glucosidase
pNP β-D-thiofucopyranoside (pH 7.5) V + – – – – – – – – + + Glucosidase
pNP β-D-mannopyranoside (pH 7.5) + + – – – – – – – – + + Glucosidase
pNP β-D-maltoside (pH 7.5) + + – – – – – – – – + + Glucosidase
pNP β-D-lactopyranoside (pH 7.5) V + – – – – – – – – + + Glucosidase
pNP β-D-glucuronide (pH 5.5) + + – – – – – – – – + – Glucosidase
Aesculin hydrolysis – – – – V – V – – – + + Classical reaction
Voges–Proskauer reaction + + – – – – – – – – + + Classical reaction
oNP β-D-galactopyranoside-6-phosphate + + – – – – – – – – + + Phosphatase
pNP phosphate-di(2-amino-2-ethyl-1,3-propanediol) (pH 5.5) + + – – – – – – – – + + Phosphatase